Instruments and Services

If you are interested in the use of our services, please contact:

nacf@uni-mainz.de

 

InstrumentServicesImage
GridIonTM
PromethION 2 SoloTM (P2S) 		Long Read Sequencing of DNA and RNA

  • Whole Genome Sequencing
    (assembly, SV- and SNV-detection, phasing)

  • Whole Transcriptome Sequencing
    (gene expression, splice variation,
    fusion transcripts, RNA-modification)

  • Epigenetics

  • Metagenomics

  • Targeted Sequencing

  • 16S Analysis
Femto Pulse SytsemHigh-sensitivity QC of DNA and RNA

  • High‑resolution qualitative and quantitative analysis of HMW gDNA up to ~160–165 kb using pulsed‑field capillary electrophoresis

  • High‑sensitivity sizing and quantification of DNA and RNA samples in picogram concentration range for NGS suitability assessment

  • QC of long‑read sequencing libraries and other DNA fragments/smears, including integrity assessment and fragment size distribution profiles

  • Assessment of RNA integrity and overall RNA quality (RIN/RQN‑like metrics) to approve samples for downstream library preparation
Fragment AnalyzerTM 5200QC of DNA and RNA

  • Sizing and quantification of gDNA and long read sequencing libraries

  • QC of NGS libraries

  • Identification of CRISPR mutation events

  • RNA integrity of total RNA


QC of mRNA/ polyA+ RNA

  • Quantification and sizing of small RNA and microRNA


Here you can find available kits

Here you can find the orderform for Fragment Analyzer runs
QubitTM4 FluorometerFluorometric Quantification of

  • dsDNA

  • ssDNA

  • RNA


Here you can find available assays
DeNovixTM DS11 PlusSpectrophotometric Quantification of

  • DNA

  • RNA

  • Cells


Quantification in microvolumes or cuvettes

Fluorometric quantification
MegaruptorTM 3Controlled Shearing of Nucleic Acids

  • Controlled fragmentation of HMW gDNA for downstream applications (e.g. PacBio, Oxford Nanopore)

  • Generation of narrow and highly reproducible DNA size ranges from ~5 kb up to >100 kb, optimized for specific library requirements

  • Homogenization of highly viscous DNA samples while keeping integrity of DNA

  • Optional QC of sheared DNA on downstream platforms (e.g. Fragment Analyzer or Femto Pulse) on request
BioruptorTMSonicator for Nucleic Acids

  • Chromatin shearing

  • DNA shearing for NGS

  • DNA extraction from FFPE samples

  • RNA shearing
CFX Opus TM96 and
CFX OpusTM 384
Real-Time PCR Detection Systems

  • 96-well, 5-Color plus FRET

  • 384-well, 4-Color plus FRET
Others

  • Enrichment of HMW DNA (CirculomicsTM)

  • Special gelelectrophoresis for HMW DNA

  • Fragmentation and size selection of DNA

  • Support with Illumina-Sequencing